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Jackson Laboratory tgf βr2 mice

Muscle-specific TGF-β signaling regulates macrophage phenotypes and efferocytosis in inflamed muscle. (A) FACS analysis of the proportion of M1 (F4/80 + Ly6C + ) and M2 (F4/80 + CD206 + ) macrophages. (B) qRT-PCR analysis of gene levels of anti-inflammatory (Arg-1, Mrc1, Retlna) and pro-inflammatory (iNOS, TNF-α, IL-6) molecules in macrophages sorted from damaged muscle of control and <t>SM</t> <t>TGF-βr2</t> -/- mice on day 3 post-myoinjury( n = 3). (C) Immunofluorescence analysis of the efferocytic macrophages (Tunel + F4/80 + ) and the free macrophages (Tunel - F4/80 + ). White arrow indicates the efferocytic macrophages. (D) FACS analysis of the proportion of F4/80 + Tunel + , and F4/80 + Tunel - macrophages. Multiple comparisons are analyzed by One-way ANOVA. Data are presented as mean ± SD ( n = 4). * p < 0.05, ** p < 0.01, *** p < 0.001. Scale bar=50 μm. ns, not significant.

Tgf βr2 Mice, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Regenerating myofiber with activating of TGF-β signaling contributes to macrophage efferocytosis through enhancing Tregs response in inflamed muscle"

Article Title: Regenerating myofiber with activating of TGF-β signaling contributes to macrophage efferocytosis through enhancing Tregs response in inflamed muscle

Journal: Frontiers in Immunology

doi: 10.3389/fimmu.2026.1810106

Figure Legend Snippet: Muscle-specific TGF-β signaling regulates macrophage phenotypes and efferocytosis in inflamed muscle. (A) FACS analysis of the proportion of M1 (F4/80 + Ly6C + ) and M2 (F4/80 + CD206 + ) macrophages. (B) qRT-PCR analysis of gene levels of anti-inflammatory (Arg-1, Mrc1, Retlna) and pro-inflammatory (iNOS, TNF-α, IL-6) molecules in macrophages sorted from damaged muscle of control and SM TGF-βr2 -/- mice on day 3 post-myoinjury( n = 3). (C) Immunofluorescence analysis of the efferocytic macrophages (Tunel + F4/80 + ) and the free macrophages (Tunel - F4/80 + ). White arrow indicates the efferocytic macrophages. (D) FACS analysis of the proportion of F4/80 + Tunel + , and F4/80 + Tunel - macrophages. Multiple comparisons are analyzed by One-way ANOVA. Data are presented as mean ± SD ( n = 4). * p < 0.05, ** p < 0.01, *** p < 0.001. Scale bar=50 μm. ns, not significant.

Techniques Used: Quantitative RT-PCR, Control, Immunofluorescence, TUNEL Assay

Muscle TGF-β signaling prompts to Tregs-mediated macrophage efferocytosis by suppressing myofiber IL-6 production. (A) Western blot and immunofluorescence analysis show IL-6 and p-STAT3 expression in inflamed muscle ( n = 3). (B) Western blot analysis of IL-6 and p-STAT3 protein levels in control or SM TGF-βr2 -/- MPC-myotubes cultured in pro-inflammatory milieu, with or without SRI administration ( n = 3). (C) FACS analysis of the proportion of CD25 + p-STAT3 + cells and CD25 + gp130 + cells in inflamed muscle ( n = 4). (D) FACS analysis of the proportion of CD25 + Foxp3 + cells and CD25 + IL-13 + cells in Tregs co-cultured with TGF-βr2 -/- or control-MPC-myotubes, treated with or without AH or SRI ( n = 3). (E) qRT-PCR analysis of Foxp3 and IL-13 mRNA levels in Tregs co-cultured with TGF-βr2 -/- or control-MPC-myotubes, treated with or without AH or SRI ( n = 3). Multiple comparisons are analyzed by One-way ANOVA. Data are presented as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001. Scale bar=50 μm. ns, not significant.

Figure Legend Snippet: Muscle TGF-β signaling prompts to Tregs-mediated macrophage efferocytosis by suppressing myofiber IL-6 production. (A) Western blot and immunofluorescence analysis show IL-6 and p-STAT3 expression in inflamed muscle ( n = 3). (B) Western blot analysis of IL-6 and p-STAT3 protein levels in control or SM TGF-βr2 -/- MPC-myotubes cultured in pro-inflammatory milieu, with or without SRI administration ( n = 3). (C) FACS analysis of the proportion of CD25 + p-STAT3 + cells and CD25 + gp130 + cells in inflamed muscle ( n = 4). (D) FACS analysis of the proportion of CD25 + Foxp3 + cells and CD25 + IL-13 + cells in Tregs co-cultured with TGF-βr2 -/- or control-MPC-myotubes, treated with or without AH or SRI ( n = 3). (E) qRT-PCR analysis of Foxp3 and IL-13 mRNA levels in Tregs co-cultured with TGF-βr2 -/- or control-MPC-myotubes, treated with or without AH or SRI ( n = 3). Multiple comparisons are analyzed by One-way ANOVA. Data are presented as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001. Scale bar=50 μm. ns, not significant.

Techniques Used: Western Blot, Immunofluorescence, Expressing, Control, Cell Culture, Quantitative RT-PCR

Myofiber TGF-β signaling regulates Tregs-mediated macrophage efferocytosis in vitro. (A) Scheme of the co-culture experiment. (B) Fluorescence staining shows DiD + ACs engulf by macrophages in co-culture system with or without TGF-βr2 -/- MPC-myotubes, SRI, or AH. (C) FACS analysis of the proportion of F4/80 + CD206 + M2 macrophages and macrophages engulfed DiD + ACs in co-culture system. (D) FACS analysis of the proportion of F4/80 + IL-10 + , F4/80 + p-STAT3 + , F4/80 + Vav1 + and F4/80 + Rac1 + cells. Multiple comparisons are analyzed by One-way ANOVA. Data are presented as mean ± SD ( n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001. Scale bar=50 μm.

Figure Legend Snippet: Myofiber TGF-β signaling regulates Tregs-mediated macrophage efferocytosis in vitro. (A) Scheme of the co-culture experiment. (B) Fluorescence staining shows DiD + ACs engulf by macrophages in co-culture system with or without TGF-βr2 -/- MPC-myotubes, SRI, or AH. (C) FACS analysis of the proportion of F4/80 + CD206 + M2 macrophages and macrophages engulfed DiD + ACs in co-culture system. (D) FACS analysis of the proportion of F4/80 + IL-10 + , F4/80 + p-STAT3 + , F4/80 + Vav1 + and F4/80 + Rac1 + cells. Multiple comparisons are analyzed by One-way ANOVA. Data are presented as mean ± SD ( n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001. Scale bar=50 μm.

Techniques Used: In Vitro, Co-Culture Assay, Fluorescence, Staining

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Journal: Frontiers in Immunology

Article Title: Regenerating myofiber with activating of TGF-β signaling contributes to macrophage efferocytosis through enhancing Tregs response in inflamed muscle

doi: 10.3389/fimmu.2026.1810106

Figure Lengend Snippet: Muscle-specific TGF-β signaling regulates macrophage phenotypes and efferocytosis in inflamed muscle. (A) FACS analysis of the proportion of M1 (F4/80 + Ly6C + ) and M2 (F4/80 + CD206 + ) macrophages. (B) qRT-PCR analysis of gene levels of anti-inflammatory (Arg-1, Mrc1, Retlna) and pro-inflammatory (iNOS, TNF-α, IL-6) molecules in macrophages sorted from damaged muscle of control and SM TGF-βr2 -/- mice on day 3 post-myoinjury( n = 3). (C) Immunofluorescence analysis of the efferocytic macrophages (Tunel + F4/80 + ) and the free macrophages (Tunel - F4/80 + ). White arrow indicates the efferocytic macrophages. (D) FACS analysis of the proportion of F4/80 + Tunel + , and F4/80 + Tunel - macrophages. Multiple comparisons are analyzed by One-way ANOVA. Data are presented as mean ± SD ( n = 4). * p < 0.05, ** p < 0.01, *** p < 0.001. Scale bar=50 μm. ns, not significant.

Article Snippet: Skeletal muscle-specific TGF-β receptor 2 knockout mice (referred to as SM TGF-βr2 −/− ) were generated by crossing MCK-Cre mice (The Jackson Laboratory) with floxed TGF-βr2 mice (TGF-βr2 flox/flox , The Jackson Laboratory).

Techniques: Quantitative RT-PCR, Control, Immunofluorescence, TUNEL Assay

Journal: Frontiers in Immunology

Article Title: Regenerating myofiber with activating of TGF-β signaling contributes to macrophage efferocytosis through enhancing Tregs response in inflamed muscle

doi: 10.3389/fimmu.2026.1810106

Figure Lengend Snippet: Muscle TGF-β signaling prompts to Tregs-mediated macrophage efferocytosis by suppressing myofiber IL-6 production. (A) Western blot and immunofluorescence analysis show IL-6 and p-STAT3 expression in inflamed muscle ( n = 3). (B) Western blot analysis of IL-6 and p-STAT3 protein levels in control or SM TGF-βr2 -/- MPC-myotubes cultured in pro-inflammatory milieu, with or without SRI administration ( n = 3). (C) FACS analysis of the proportion of CD25 + p-STAT3 + cells and CD25 + gp130 + cells in inflamed muscle ( n = 4). (D) FACS analysis of the proportion of CD25 + Foxp3 + cells and CD25 + IL-13 + cells in Tregs co-cultured with TGF-βr2 -/- or control-MPC-myotubes, treated with or without AH or SRI ( n = 3). (E) qRT-PCR analysis of Foxp3 and IL-13 mRNA levels in Tregs co-cultured with TGF-βr2 -/- or control-MPC-myotubes, treated with or without AH or SRI ( n = 3). Multiple comparisons are analyzed by One-way ANOVA. Data are presented as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001. Scale bar=50 μm. ns, not significant.

Techniques: Western Blot, Immunofluorescence, Expressing, Control, Cell Culture, Quantitative RT-PCR

Journal: Frontiers in Immunology

Article Title: Regenerating myofiber with activating of TGF-β signaling contributes to macrophage efferocytosis through enhancing Tregs response in inflamed muscle

doi: 10.3389/fimmu.2026.1810106

Figure Lengend Snippet: Myofiber TGF-β signaling regulates Tregs-mediated macrophage efferocytosis in vitro. (A) Scheme of the co-culture experiment. (B) Fluorescence staining shows DiD + ACs engulf by macrophages in co-culture system with or without TGF-βr2 -/- MPC-myotubes, SRI, or AH. (C) FACS analysis of the proportion of F4/80 + CD206 + M2 macrophages and macrophages engulfed DiD + ACs in co-culture system. (D) FACS analysis of the proportion of F4/80 + IL-10 + , F4/80 + p-STAT3 + , F4/80 + Vav1 + and F4/80 + Rac1 + cells. Multiple comparisons are analyzed by One-way ANOVA. Data are presented as mean ± SD ( n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001. Scale bar=50 μm.

Techniques: In Vitro, Co-Culture Assay, Fluorescence, Staining

Primer sequences used for phenotype identification of SM TGF-βr2 −/− mice

Journal: Cell Communication and Signaling : CCS

Article Title: Myofiber directs macrophages IL-10-Vav1-Rac1 efferocytosis pathway in inflamed muscle following CTX myoinjury by activating the intrinsic TGF-β signaling

doi: 10.1186/s12964-023-01163-8

Figure Lengend Snippet: Primer sequences used for phenotype identification of SM TGF-βr2 −/− mice

Article Snippet: Mice with TGF-βr2 knockout in skeletal muscle (designated as ‘SM TGF-βr2 −/− mice’) were generated by crossing MCK-Cre mice (Jackson Lab) with floxed TGF-βr2 (TGF-βr2 flox/flox , Jackson Lab).

Techniques:

Journal: Cell Communication and Signaling : CCS

Article Title: Myofiber directs macrophages IL-10-Vav1-Rac1 efferocytosis pathway in inflamed muscle following CTX myoinjury by activating the intrinsic TGF-β signaling

doi: 10.1186/s12964-023-01163-8

Figure Lengend Snippet: Primer sequences used for PCR

Techniques:

The inflammatory stimulus drives the myofiber specific TGF-β signal activation. A , RNA-seq analysis showing the mRNA level changes of TGF-β2 and TGF-βr2 of inflamed TA muscle. B , The mRNA levels of TGF-β2 and TGF-βr2 of inflamed muscle were quantified by qRT-PCR. C , Western blot analysis of protein levels in TGF-β2, p-TGF-βr2 and p-Smad2/3 in inflamed muscle. The relative protein level values were expressed as a ratio (protein of interest/GAPDH or phosphorylated (p) protein/total protein). D , Immunofluorescence double-staining results of TGF-βr2, p-TGF-βr2, p-Smad2/3 and Dystrophin in inflamed muscle. E , Western blot analysis of the protein levels of TGF-β2, p-TGF-βr2 and p-Smad2/3 in MPC-Myotubes receiving pro-inflammatory administration. Statistical data were expressed as the mean ± SD ( n = 3). Multiple comparisons were analyzed by One-way ANOVA (* P < 0.05, ** P < 0.01). Bar = 50 μm

Journal: Cell Communication and Signaling : CCS

Article Title: Myofiber directs macrophages IL-10-Vav1-Rac1 efferocytosis pathway in inflamed muscle following CTX myoinjury by activating the intrinsic TGF-β signaling

doi: 10.1186/s12964-023-01163-8

Figure Lengend Snippet: The inflammatory stimulus drives the myofiber specific TGF-β signal activation. A , RNA-seq analysis showing the mRNA level changes of TGF-β2 and TGF-βr2 of inflamed TA muscle. B , The mRNA levels of TGF-β2 and TGF-βr2 of inflamed muscle were quantified by qRT-PCR. C , Western blot analysis of protein levels in TGF-β2, p-TGF-βr2 and p-Smad2/3 in inflamed muscle. The relative protein level values were expressed as a ratio (protein of interest/GAPDH or phosphorylated (p) protein/total protein). D , Immunofluorescence double-staining results of TGF-βr2, p-TGF-βr2, p-Smad2/3 and Dystrophin in inflamed muscle. E , Western blot analysis of the protein levels of TGF-β2, p-TGF-βr2 and p-Smad2/3 in MPC-Myotubes receiving pro-inflammatory administration. Statistical data were expressed as the mean ± SD ( n = 3). Multiple comparisons were analyzed by One-way ANOVA (* P < 0.05, ** P < 0.01). Bar = 50 μm

Techniques: Activation Assay, RNA Sequencing, Quantitative RT-PCR, Western Blot, Immunofluorescence, Double Staining

Endogenous TGF-β signaling regulates IL-10 production in regenerating myofiber. Transcriptome assay ( A ), qRT-PCR analysis ( B ) and Luminex analysis ( C ) of gene levels of some myokines, mRNA level of IL-10, protein levels of some myokines in TGF-βr2 −/− - or control-MPC-Myotubes exposed to pro-inflammatory milieu, respectively. qRT-PCR analysis ( D ) of IL-10 mRNA level in inflamed muscle. Immunofluorescence staining ( E ) showed IL-10 expression change in regenerating myofibers. Multiple comparisons were analyzed by One-way ANOVA. Statistical data were expressed as mean ± SD ( n = 3). (* P < 0.05, ** P < 0.01). Bar = 50 μm

Journal: Cell Communication and Signaling : CCS

Article Title: Myofiber directs macrophages IL-10-Vav1-Rac1 efferocytosis pathway in inflamed muscle following CTX myoinjury by activating the intrinsic TGF-β signaling

doi: 10.1186/s12964-023-01163-8

Figure Lengend Snippet: Endogenous TGF-β signaling regulates IL-10 production in regenerating myofiber. Transcriptome assay ( A ), qRT-PCR analysis ( B ) and Luminex analysis ( C ) of gene levels of some myokines, mRNA level of IL-10, protein levels of some myokines in TGF-βr2 −/− - or control-MPC-Myotubes exposed to pro-inflammatory milieu, respectively. qRT-PCR analysis ( D ) of IL-10 mRNA level in inflamed muscle. Immunofluorescence staining ( E ) showed IL-10 expression change in regenerating myofibers. Multiple comparisons were analyzed by One-way ANOVA. Statistical data were expressed as mean ± SD ( n = 3). (* P < 0.05, ** P < 0.01). Bar = 50 μm

Techniques: Quantitative RT-PCR, Luminex, Control, Immunofluorescence, Staining, Expressing

Myofiber TGF-β-IL-10 signaling effects on macrophages efferocytosis. Immunofluorescence staining (upper part) and FACS analysis (lower part) of the uptake of PKH67 labeled apoptotic cells by macrophage co-cultured with TGF-βr2 −/− - or control-MPC-Myotubes exposed to pro-inflammatory milieu, and treated with or without SRI, rmIL-10 or AS101. Mac: Macrophage; ACs: Apoptotic cells; Multiple comparisons were analyzed by One-way ANOVA. Statistical data were expressed as mean ± SD ( n = 3). (* P < 0.05, ** P < 0.01). Bar = 50 μm

Journal: Cell Communication and Signaling : CCS

Article Title: Myofiber directs macrophages IL-10-Vav1-Rac1 efferocytosis pathway in inflamed muscle following CTX myoinjury by activating the intrinsic TGF-β signaling

doi: 10.1186/s12964-023-01163-8

Figure Lengend Snippet: Myofiber TGF-β-IL-10 signaling effects on macrophages efferocytosis. Immunofluorescence staining (upper part) and FACS analysis (lower part) of the uptake of PKH67 labeled apoptotic cells by macrophage co-cultured with TGF-βr2 −/− - or control-MPC-Myotubes exposed to pro-inflammatory milieu, and treated with or without SRI, rmIL-10 or AS101. Mac: Macrophage; ACs: Apoptotic cells; Multiple comparisons were analyzed by One-way ANOVA. Statistical data were expressed as mean ± SD ( n = 3). (* P < 0.05, ** P < 0.01). Bar = 50 μm

Techniques: Immunofluorescence, Staining, Labeling, Cell Culture, Control

Myofiber TGF-β-IL-10 signaling effects on macrophage phenotype and efferocytosis related-molecules expression. FACS analysis of Bcl3, p-STAT3, Vav1, GTPase Rac1, CD206 and CX3CR1 in macrophages co-cultured with TGF-βr2. −/− - or control-MPC-Myotubes, exposed to pro-inflammatory milieu, and treated with or without SRI, rmIL-10 or AS101, respectively. Multiple comparisons were analyzed by One-way ANOVA. Statistical data were expressed as mean ± SD ( n = 3). (* P < 0.05, ** P < 0.01)

Journal: Cell Communication and Signaling : CCS

Article Title: Myofiber directs macrophages IL-10-Vav1-Rac1 efferocytosis pathway in inflamed muscle following CTX myoinjury by activating the intrinsic TGF-β signaling

doi: 10.1186/s12964-023-01163-8

Figure Lengend Snippet: Myofiber TGF-β-IL-10 signaling effects on macrophage phenotype and efferocytosis related-molecules expression. FACS analysis of Bcl3, p-STAT3, Vav1, GTPase Rac1, CD206 and CX3CR1 in macrophages co-cultured with TGF-βr2. −/− - or control-MPC-Myotubes, exposed to pro-inflammatory milieu, and treated with or without SRI, rmIL-10 or AS101, respectively. Multiple comparisons were analyzed by One-way ANOVA. Statistical data were expressed as mean ± SD ( n = 3). (* P < 0.05, ** P < 0.01)

Techniques: Expressing, Cell Culture, Control

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